以DNA为基础的HPA-1到-17系统测序分型
发布时间：2010年09月13日
来源：南宁输血医学研究所血小板免疫学网 阅读次数：
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| 作者：Wu GG, Tang QM, Shen WD, Liao Y, Li HC, Zhao TM.来源：Int J Hematol. 2008 Oct;88(3):268-71.摘要：虽然已经建立了PCR-SSP和PCR-SSO等几种以DNA为基础的人类血小板特异性抗原的分型方法，但分型的错误和缺乏实验室间的可重复性仍然是关键问题。在目前的研究中，设计不同的PCR引物来鉴定所有不同表型的HPA-1～-17w，用基因组DNA样本的基因测序分型方法。PCR-SSP和SBT对HPA已知型的血小板供者和罕见的HPA-1bb，2bb和6bb纯合子等供者的分型结果是一致的。 DNA sequencing-based typing of HPA-1 to HPA-17w systemsWu GG, Tang QM, Shen WD, Liao Y, Li HC, Zhao TM.Int J Hematol. 2008 Oct;88(3):268-71.Abstract: Although several DNA-based human platelet antigens (HPA) typing techniques, such as PCR-SSP and PCR-SSO, have been established, the typing errors and the lack of interlaboratory reproducibility are still the issues of concerns. In the present study, polymerase chain reaction primers were designed for identification of all the phenotypically different HPA-1 to HPA-17w types by sequencing-based typing (SBT) method using genomic DNA samples. No discrepancies were observed between PCR-SSP typing and SBT typing in typing a panel of HPA-typed platelet donors that included all common HPA types and the rare HPA-1b, 2b, 3b, and 6bw homozygous donors. 本栏目负责人：黎海燕 |
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