作者：申卫东, 周燕, 钟周琳, 唐秋民, 莫秋红, 李恒聪, 何保仁, 黎海燕, 廖燕,  李丽兰, 吴国光

来源：中国输血杂志, 2009, 22(9): 719-721

目的：主持国际输血学会第14届国际血小板免疫学研讨会和合作研究项目,对参加合作研究项目的全球42个血小板免疫学实验室的血小板抗体检测技术和质量状况进行测评。

方法：南宁输血医学研究所组织并向参加合作研究的各实验室提供9份含抗血小板特异性抗体的质控标本,各实验室以本室的技术或市场化的技术作检测,按所提供的记录表格对各质控标本的反应结果作报告。

结果：23个国家的36个实验室参加了本合作项目,35个实验室反馈了各自实验室的研究结果,9个质控标本抗体特异性鉴定的一致率为20%—97.14%,抗体特异性分别为抗-HPA-1a、抗-HPA-1b、抗-HPA-3a、抗-HPA-3a、抗-HPA-3b、抗-HPA-5b、抗-HPA-5a、抗-GPIV及抗-HPA-5b+15b,一致率最低为抗-HPA-3b,最高为抗-HPA-5b和抗-HPA-5a。各实验室采用的技术共计有10余种,血小板抗原单克隆抗体特异性免疫固定检测技术(MAIPA)是采用最多的技术。

结论：本合作项目成功地对35个国际血小板免疫诊断实验室的血小板特异性抗体的检测技术和质量进行了测评和分析报告。

Proficiency evaluation of platelet alloantiboby analysis in 42 platelet Immunology laboratories around the world:the 14th ISBT Platelet Immunology Workshop and Cooperative Research Project

SHEN Weidong; ZHOU Yan; ZHONG ZhouLin; WU GuoGuang

Chin J Blood Transfusion, 2009, 22(9): 719-721

Objective: To organize the 14th ISBT Platelet Immunology Workshop and Cooperative Research Project,and proficiency evaluation on the techniques and quality level of platelet alloantiboby analysis will be taken effect for the 42 platelet immunology laboratories around the world.

Methods: Organized and confirmed by Nanning Institute of Transfusion Medicine,9 quality control samples contained the Human Platelet Antigen (HPA) specificity antibody have been supplied to all the participated laboratories in this project. The participants could use the In-House technology or/and market kits to test these quality control samples. The forms and sheets have been provided for recording of results.

Results: There were 36 laboratories from 23 countries participated in this Cooperative Research Project,and 35 laboratories have reported their results. The appraised consistent rate of the 9 quality control samples ranges from 20% to 97.14%,the HPA antibody specificities have been showed as anti HPA-1a,anti HPA-1b,anti HPA-3a,anti HPA-3a,anti HPA-3b,anti HPA-5b,anti HPA-5a,anti GPIV and anti HPA-5b+15b,respectively. Among the appraised consistent rate,the anti HPA-3b were the lowest and the anti HPA-5b and anti HPA-5a was the highest. Over 10 technologies of platelet alloantiboby analysis were used by the laboratories.

Conclusion: This international cooperative research project has successfully made the proficiency evaluation and report on the techniques and quality level of platelet alloantiboby analysis for the 35 international platelet immunology laboratories.

本栏目负责人：黎海燕