作者：周燕  刘金莲  钟周琳  李丽兰 申卫东 吴国光

 来源：临床血液学杂志, 2015,28(6):459-461.

[摘要]  目的：建立流式细胞术筛查血小板抗体方法应用于临床检测。方法：对78例需要进行血小板抗体检测的病例作流式细胞筛查术检测，同时采用经典酶联免疫吸附实验（PAKPLUS试剂盒）做对照，比较两种方法的实验结果。结果：流式细胞术的血小板抗体检出率为35.90%（28/78），PAKPLUS的检出率为25.65%（20/78），两种方法阳性率差异无统计学意义（P＞0.05）；经MAIPA技术鉴定，流式细胞术筛查血小板抗体为阳性的患者中，有10例含抗HLA抗体，有1例含抗HPA-5b抗体，有9例为自身抗体。结论：成功建立了流式细胞术筛查血小板抗体并应用于临床检测；该技术拥有快速、易于操作的特点，且能实现程序化、标准化检测，值得推广应用。

[关键词]血小板抗体；筛查；流式细胞术

Establishment and application of experiment method for Flow Cytometry of platelet antibody screening

ZHOU Yan, LIU Jin-Llian, ZHONG Zhou-Lin, LI Li-Lan, SHEN Wei-Dong, WU Guo-Gang
Journal of Clinical Hematology, 2015,28(6):459-461.

Abstract  Objective：To establish platelet antibody screening using Flow Cytometry(FCM) and apply to the clinic. Methods: The platelet antibody in 78 patients were detected by FCM and PAKPLUS, respectively. The results between two different techniques were compared. Results: In 78 patients , 28 were positive in FCM（35.90%）,20 were positive in PAKPLUS（25.65%）, there was no significant difference between two methods（P＞0.05）. After identification by MAIPA, 10 patients were anti-HLA, one patient were anti-HPA-5b, 9 patients were autoantibody. Conclusion: We established FCM technique to screen platelet antibody successfully. FCM may become the standard platelet antibody screening method as a possible alternative to be recommended. 
Key words:  platelet antibody; screen; Flow Cytometry;