作者：周燕，钟周琳，刘金莲

来源：国际检验医学杂志, 2015, 36(11): 1500-1504.

    摘要：目的 研究血小板膜糖蛋白特异性自身抗体在成人及儿童原发免疫性血小板减少症（ITP）患者中分布的异同。方法 应用酶联免疫吸附法（PAKAUTO试剂盒）检测ITP组（83例）及非ITP组（58例）患者血小板自身抗体，并分析成人组ITP（43例），儿童组ITP（37例）的抗GPIIb/IIIa、抗GPIb/IX及抗GPIa/IIa自身抗体特异性分布规律。结果 ITP组血小板自身抗体阳性率为66.27%，高于非ITP组的6.90%,差异有统计学意义差异有统计学意义（P＜0.05）。成人组女性ITP患者占60.87%，儿童组女性ITP患者占64.86%，差异无统计学意义（P＞0.05），但两组女性发病率均高于男性，差异有统计学意义（P＜0.05）。成人组ITP患者血小板自身抗体阳性率为63.04%，儿童组ITP患者抗体阳性率为70.27%，差异无统计学意义（P＞0.05）；且成人组与儿童组ITP患者血小板自身抗体特异性分布差异无统计学意义（P＞0.05），均以抗GPIIb/IIIa和抗GPIb/IX抗体多见。结论 血小板自身抗体检测对ITP诊断、鉴别诊断及治疗均有重要的参考价值，GPIa/IIa抗体介导的ITP很值得深入研究。

关键词：血小板减少；血小板膜糖蛋白；自身抗体；分布

Study on the distribution of autoantibodies against platelet in adults and chidren with primary immune thrombocytopenia in Nanning

Zhou Yan, Zhong Zhoulin, Liu Jinlian
International Journal of Laboratory Medicine. 2015, 36(11): 1500-1504.

    Abstract:Objective  Study on the distribution of autoantibodies against platelet in adults and chidren with primary immune thrombocytopenia(ITP). Methods Platelet autoantibodies were detected in 83 ITP patients and 58 non-ITP patients by enzyme linked immunosorbent assay (PAKAUTO kit). Anti-GPIIb/IIIa,GPIb/IX and GPIa/IIa autoantibodies were analyzed for 43 adults and 37 children with ITP. Results The positive rate of autoantibody was 66.27% in ITP patients, which was much higher than that of non-ITP patients,the difference was significant（P＜0.05）. As for ITP patients, female ITP patients in adult group were 28, while in children group were 24,the difference was not significant(P＞0.05)，but the morbidity of ITP in females was higher than that of males in both of the two groups,the difference was significant（P＜0.05）. There was no significant difference on the distribution of platelet autoantibodies in adult and children group（P＞0.05）,the main of them was resistance against GPIIb/IIIa and GPIb/IX antibodies. Conclusion The detection of platelet autoantibodies could distinguish ITP and non-ITP patients,and GPIa/IIahas has an improtant reference value about therapy,which should explore more. 
Key words: thrombocytopenia; platelet membrance glycoprotein; autoantibody; distribution